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csf2 elisa  (R&D Systems)


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    R&D Systems csf2 elisa
    Csf2 Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/csf2+elisa/Mouse+GM-CSF+Quantikine+ELISA+Kit/pm41858055-262-13-15
    Average 94 stars, based on 46 article reviews
    csf2 elisa - by Bioz Stars, 2026-09
    94/100 stars

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    Concentration Assay:

    Article Title: Inflammatory Mediators Both Directly and Indirectly Promote Microglial Proliferation.
    Article Snippet: To determine the proteins present in microglia conditioned media following CSF2 stimulation, we stimulated cultured microglia with CSF2 (Gibco 315- 03, 10 ng/mL) and collected media after 72 h, centrifuged down debris at 3000 g for 10 min at 4°C before storage at −80°C until we sent three batches to Eve Technologies (Calgary, Alberta) for analysis in the Mouse Cytokine 36- Plex Discovery Assay. .. To determine the concentration of CSF2 in our concentrated ACM, we performed a CSF2 ELISA (R&D Systems MGM00) as per the manufacturer's instructions. ..

    Article Title: Inflammatory Mediators Both Directly and Indirectly Promote Microglial Proliferation
    Article Snippet: To determine the proteins present in microglia conditioned media following CSF2 stimulation, we stimulated cultured microglia with CSF2 (Gibco 315‐03, 10 ng/mL) and collected media after 72 h, centrifuged down debris at 3000 g for 10 min at 4°C before storage at −80°C until we sent three batches to Eve Technologies (Calgary, Alberta) for analysis in the Mouse Cytokine 36‐Plex Discovery Assay. .. To determine the concentration of CSF2 in our concentrated ACM, we performed a CSF2 ELISA (R&D Systems MGM00) as per the manufacturer's instructions. .. On the day of the assay, we performed a DC protein assay (Bio‐Rad 5,000,112) on each concentrated ACM sample to be tested.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Inflammatory Mediators Both Directly and Indirectly Promote Microglial Proliferation.
    Article Snippet: To determine the proteins present in microglia conditioned media following CSF2 stimulation, we stimulated cultured microglia with CSF2 (Gibco 315- 03, 10 ng/mL) and collected media after 72 h, centrifuged down debris at 3000 g for 10 min at 4°C before storage at −80°C until we sent three batches to Eve Technologies (Calgary, Alberta) for analysis in the Mouse Cytokine 36- Plex Discovery Assay. .. To determine the concentration of CSF2 in our concentrated ACM, we performed a CSF2 ELISA (R&D Systems MGM00) as per the manufacturer's instructions. ..

    Article Title: Inflammatory Mediators Both Directly and Indirectly Promote Microglial Proliferation
    Article Snippet: To determine the proteins present in microglia conditioned media following CSF2 stimulation, we stimulated cultured microglia with CSF2 (Gibco 315‐03, 10 ng/mL) and collected media after 72 h, centrifuged down debris at 3000 g for 10 min at 4°C before storage at −80°C until we sent three batches to Eve Technologies (Calgary, Alberta) for analysis in the Mouse Cytokine 36‐Plex Discovery Assay. .. To determine the concentration of CSF2 in our concentrated ACM, we performed a CSF2 ELISA (R&D Systems MGM00) as per the manufacturer's instructions. .. On the day of the assay, we performed a DC protein assay (Bio‐Rad 5,000,112) on each concentrated ACM sample to be tested.



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    Fig. 6 <t>CSF2</t> is a downstream gene of circPRKCA in TE1 and KYSE30 cells. A Differentially expressed genes in TE1 cells transfected with si-circPRKCA were identified using mRNA sequencing. B CSF2 was identified in both the circPRKCA mRNA sequencing dataset and the YBX1-related gene dataset from the TCGA database. C, D The expression of CSF2 in ESCC cells treated with si-NC/si-circPRKCA and pLC5/circPRKCA were measureed by RT-qPCR. E–G Rescue experiments confirmed the changes in scratch healing, transwell migration, and matrigel invasion capabilities of KYSE150 cells under conditions of exogenously added rhCSF2 or the knockdown of circPRKCA, as well as the angiogenic potential of HUVEC cells cultured with medium from KYSE150 cells. H, I RT-qPCR and <t>ELISA</t> were employed to assess the expression of CSF2 in ESCC cells modulated with circPRKCA or si-YBX1. * p < 0.05, ** p < 0.01, *** p < 0.001
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    Figure 9. Effect of lactobacilli strains on the production of inflammatory cytokines and chemokines by murine Peyer’s patches macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysaccharide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticaseibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, Peyer’s patches macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by <t>ELISA.</t> Peyer’s patches of macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.
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    Fig. 6 CSF2 is a downstream gene of circPRKCA in TE1 and KYSE30 cells. A Differentially expressed genes in TE1 cells transfected with si-circPRKCA were identified using mRNA sequencing. B CSF2 was identified in both the circPRKCA mRNA sequencing dataset and the YBX1-related gene dataset from the TCGA database. C, D The expression of CSF2 in ESCC cells treated with si-NC/si-circPRKCA and pLC5/circPRKCA were measureed by RT-qPCR. E–G Rescue experiments confirmed the changes in scratch healing, transwell migration, and matrigel invasion capabilities of KYSE150 cells under conditions of exogenously added rhCSF2 or the knockdown of circPRKCA, as well as the angiogenic potential of HUVEC cells cultured with medium from KYSE150 cells. H, I RT-qPCR and ELISA were employed to assess the expression of CSF2 in ESCC cells modulated with circPRKCA or si-YBX1. * p < 0.05, ** p < 0.01, *** p < 0.001

    Journal: Journal of translational medicine

    Article Title: CircPRKCA facilitates esophageal squamous cell carcinoma metastasis via m5C-dependent CSF2 mRNA stabilization.

    doi: 10.1186/s12967-025-06395-5

    Figure Lengend Snippet: Fig. 6 CSF2 is a downstream gene of circPRKCA in TE1 and KYSE30 cells. A Differentially expressed genes in TE1 cells transfected with si-circPRKCA were identified using mRNA sequencing. B CSF2 was identified in both the circPRKCA mRNA sequencing dataset and the YBX1-related gene dataset from the TCGA database. C, D The expression of CSF2 in ESCC cells treated with si-NC/si-circPRKCA and pLC5/circPRKCA were measureed by RT-qPCR. E–G Rescue experiments confirmed the changes in scratch healing, transwell migration, and matrigel invasion capabilities of KYSE150 cells under conditions of exogenously added rhCSF2 or the knockdown of circPRKCA, as well as the angiogenic potential of HUVEC cells cultured with medium from KYSE150 cells. H, I RT-qPCR and ELISA were employed to assess the expression of CSF2 in ESCC cells modulated with circPRKCA or si-YBX1. * p < 0.05, ** p < 0.01, *** p < 0.001

    Article Snippet: Cell culture supernatants were collected and analyzed for cytokine production using a Human CSF2 ELISA kit (Proteintech, Wuhan, China) according to the manufacturer’s protocol.

    Techniques: Transfection, Sequencing, Expressing, Quantitative RT-PCR, Migration, Knockdown, Cell Culture, Enzyme-linked Immunosorbent Assay

    Fig. 7 CircPRKCA/YBX1 enhances the stability of CSF2 mRNA through m5C modification in KYSE30 and KYSE150 cells. A RIP assay verifies the interaction between YBX1 and CSF2. B MeRIP assay confirms the presence of m5C methylation sites in CSF2. C, D Actinomycin D experiments assess the regulation of CSF2 mRNA stability by YBX1. E, F A MeRIP experiment verifies the changes in CSF2 mRNA enrichment levels induced by the m5C antibody in ESCC cells transfected with circPRKCA or si-YBX1. G, H Salvage experiments confirm the effect of actinomycin D on the stabilization of CSF2 mRNA in ESCC cells regulated by circPRKCA or si-YBX1. I, J. The levels of YBX1, CSF2, CD31, and CD34 in the pLC5 and circPRKCA vector groups were detected by IHC. K The mechanism of the circPRKCA/YBX1/CSF2 axis in promoting the progression of ESCC metastasis. *p < 0.05, **p < 0.01, ***p < 0.001

    Journal: Journal of translational medicine

    Article Title: CircPRKCA facilitates esophageal squamous cell carcinoma metastasis via m5C-dependent CSF2 mRNA stabilization.

    doi: 10.1186/s12967-025-06395-5

    Figure Lengend Snippet: Fig. 7 CircPRKCA/YBX1 enhances the stability of CSF2 mRNA through m5C modification in KYSE30 and KYSE150 cells. A RIP assay verifies the interaction between YBX1 and CSF2. B MeRIP assay confirms the presence of m5C methylation sites in CSF2. C, D Actinomycin D experiments assess the regulation of CSF2 mRNA stability by YBX1. E, F A MeRIP experiment verifies the changes in CSF2 mRNA enrichment levels induced by the m5C antibody in ESCC cells transfected with circPRKCA or si-YBX1. G, H Salvage experiments confirm the effect of actinomycin D on the stabilization of CSF2 mRNA in ESCC cells regulated by circPRKCA or si-YBX1. I, J. The levels of YBX1, CSF2, CD31, and CD34 in the pLC5 and circPRKCA vector groups were detected by IHC. K The mechanism of the circPRKCA/YBX1/CSF2 axis in promoting the progression of ESCC metastasis. *p < 0.05, **p < 0.01, ***p < 0.001

    Article Snippet: Cell culture supernatants were collected and analyzed for cytokine production using a Human CSF2 ELISA kit (Proteintech, Wuhan, China) according to the manufacturer’s protocol.

    Techniques: Modification, Methylation, Transfection, Plasmid Preparation

    Figure 9. Effect of lactobacilli strains on the production of inflammatory cytokines and chemokines by murine Peyer’s patches macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysaccharide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticaseibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, Peyer’s patches macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by ELISA. Peyer’s patches of macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

    Journal: International journal of molecular sciences

    Article Title: Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506.

    doi: 10.3390/ijms26062688

    Figure Lengend Snippet: Figure 9. Effect of lactobacilli strains on the production of inflammatory cytokines and chemokines by murine Peyer’s patches macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysaccharide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticaseibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, Peyer’s patches macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by ELISA. Peyer’s patches of macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

    Article Snippet: 2025, 26, 2688 22 of 26 BMS614) and IL-12 (Mouse IL-12 p70 ELISA Kit, BMS6004) from ThermoFisher Scientific (Waltham, MA, USA), and CCL-2 (Mouse CCL2/JE/MCP-1 ELISA Kit Quantikine, MJE00B), CCL-8 (Mouse CCL8/MCP-2 DuoSet ELISA, DY790), IL-27 (Mouse IL-27 p28/IL-30 ELISA Kit Quantikine, M2728), CSF2 (Mouse GM-CSF DuoSet ELISA, DY415), and CSF3 (Mouse G-CSF ELISA Kit Quantikine, MCS00) from R&D Systems (Minneapolis, MN, USA).

    Techniques: Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay

    Figure 10. Effect of lactobacilli strains on the production of inflammatory and regulatory cytokines by murine Peyer’s patches macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysaccharide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticaseibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, Peyer’s patches macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by ELISA. Peyer’s patches macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

    Journal: International journal of molecular sciences

    Article Title: Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506.

    doi: 10.3390/ijms26062688

    Figure Lengend Snippet: Figure 10. Effect of lactobacilli strains on the production of inflammatory and regulatory cytokines by murine Peyer’s patches macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysaccharide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticaseibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, Peyer’s patches macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by ELISA. Peyer’s patches macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

    Article Snippet: 2025, 26, 2688 22 of 26 BMS614) and IL-12 (Mouse IL-12 p70 ELISA Kit, BMS6004) from ThermoFisher Scientific (Waltham, MA, USA), and CCL-2 (Mouse CCL2/JE/MCP-1 ELISA Kit Quantikine, MJE00B), CCL-8 (Mouse CCL8/MCP-2 DuoSet ELISA, DY790), IL-27 (Mouse IL-27 p28/IL-30 ELISA Kit Quantikine, M2728), CSF2 (Mouse GM-CSF DuoSet ELISA, DY415), and CSF3 (Mouse G-CSF ELISA Kit Quantikine, MCS00) from R&D Systems (Minneapolis, MN, USA).

    Techniques: Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay

    Figure 11. Effect of lactobacilli strains on the production of inflammatory cytokines and chemokines by murine peritoneal macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysac- charide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticas- eibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, peritoneal macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by ELISA. Peritoneal macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

    Journal: International journal of molecular sciences

    Article Title: Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506.

    doi: 10.3390/ijms26062688

    Figure Lengend Snippet: Figure 11. Effect of lactobacilli strains on the production of inflammatory cytokines and chemokines by murine peritoneal macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysac- charide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticas- eibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, peritoneal macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by ELISA. Peritoneal macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

    Article Snippet: 2025, 26, 2688 22 of 26 BMS614) and IL-12 (Mouse IL-12 p70 ELISA Kit, BMS6004) from ThermoFisher Scientific (Waltham, MA, USA), and CCL-2 (Mouse CCL2/JE/MCP-1 ELISA Kit Quantikine, MJE00B), CCL-8 (Mouse CCL8/MCP-2 DuoSet ELISA, DY790), IL-27 (Mouse IL-27 p28/IL-30 ELISA Kit Quantikine, M2728), CSF2 (Mouse GM-CSF DuoSet ELISA, DY415), and CSF3 (Mouse G-CSF ELISA Kit Quantikine, MCS00) from R&D Systems (Minneapolis, MN, USA).

    Techniques: Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay

    Figure 12. Effect of lactobacilli strains on the production of inflammatory and regulatory cytokines by murine peritoneal macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysac- charide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticas- eibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, peritoneal macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by ELISA. Peritoneal macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

    Journal: International journal of molecular sciences

    Article Title: Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506.

    doi: 10.3390/ijms26062688

    Figure Lengend Snippet: Figure 12. Effect of lactobacilli strains on the production of inflammatory and regulatory cytokines by murine peritoneal macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysac- charide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticas- eibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, peritoneal macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by ELISA. Peritoneal macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

    Article Snippet: 2025, 26, 2688 22 of 26 BMS614) and IL-12 (Mouse IL-12 p70 ELISA Kit, BMS6004) from ThermoFisher Scientific (Waltham, MA, USA), and CCL-2 (Mouse CCL2/JE/MCP-1 ELISA Kit Quantikine, MJE00B), CCL-8 (Mouse CCL8/MCP-2 DuoSet ELISA, DY790), IL-27 (Mouse IL-27 p28/IL-30 ELISA Kit Quantikine, M2728), CSF2 (Mouse GM-CSF DuoSet ELISA, DY415), and CSF3 (Mouse G-CSF ELISA Kit Quantikine, MCS00) from R&D Systems (Minneapolis, MN, USA).

    Techniques: Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay